rt-qpcr and western blot analyses of caspase-3, cleaved caspase valproate pretreatment protects pancreatic β-cells apoptosis western blot cocktail (pro/p17-caspase 3, cleaved-parp cleaved caspase-3 (asp175) antibody (a) immunohistochemical analysis of caspase-3, cleaved caspase-3